Hotspot

rpl32-trnL-CUA

SSC · 113025-113846 · 822 bp

Back to Rhammatophyllum

Pi 0.024

MarkerSeek score 71.30

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 822
variable_sites: 28
pi: 0.024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	916-956 bp	1.0	71.2
rpl32-trnL-CUA_p2	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	909-949 bp	1.0	70.8
rpl32-trnL-CUA_p3	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	915-955 bp	1.0	70.7
rpl32-trnL-CUA_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	914-954 bp	1.0	70.7
rpl32-trnL-CUA_p5	GGAAGGGATATTGGACATCGT	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.2	58.3	910-950 bp	1.0	70.1