Hotspot

ndhC-trnV-GUA

LSC · 49763-50643 · 881 bp

Back to Rhammatophyllum

Pi 0.0301
MarkerSeek score 68.90
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
881
variable_sites
35
pi
0.0301
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 31.8 55.0 53.9 58.7 843-943 bp 1.0 47.1
ndhC-trnV-GUA_p2 TCATATTCGTAAAGCAGAAACA TTCGAGTCCGTATAGCCCTA 31.8 50.0 53.9 57.1 842-942 bp 1.0 47.1
ndhC-trnV-GUA_p3 TCATATTCGTAAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 30.4 55.0 54.5 58.7 843-943 bp 1.0 47.1
ndhC-trnV-GUA_p4 TCATATTCGTAAAGCAGAAACA TCGAACTCTTTTACCGAGCAG 31.8 47.6 53.9 58.1 871-971 bp 1.0 47.0
ndhC-trnV-GUA_p5 TCATATTCGTAAAGCAGAAACA TCGAACTCTTTTACCGAGCA 31.8 45.0 53.9 56.9 871-971 bp 1.0 47.0