Hotspot

ndhK-ndhC

LSC · 49336-49399 · 64 bp

Back to Rhammatophyllum

Pi 0.0376

MarkerSeek score 79.40

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 3
pi: 0.0376
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TAAAGAAGCGGCCATAAGCT	ATGGCGAAAGGGAGCATTAG	45.0	50.0	57.3	58.0	202-211 bp	1.0	78.7
ndhK-ndhC_p2	TAAAGAAGCGGCCATAAGCT	GGCGAAAGGGAGCATTAGAA	45.0	50.0	57.3	58.0	200-209 bp	1.0	78.7
ndhK-ndhC_p3	TAAAGAAGCGGCCATAAGCT	TTTATGCATGGCGAAAGGGA	45.0	45.0	57.3	57.9	209-218 bp	1.0	77.7
ndhK-ndhC_p4	TAAAGAAGCGGCCATAAGCT	GTTTATGCATGGCGAAAGGG	45.0	50.0	57.3	57.8	210-219 bp	1.0	77.1
ndhK-ndhC_p5	TAAAGAAGCGGCCATAAGCT	TGGGCAATGAGTTTCGATGT	45.0	45.0	57.3	57.8	291-300 bp	1.0	76.0