Hotspot

trnT-ACC-psbD

LSC · 30856-32143 · 1288 bp

Back to Rhammatophyllum

Pi 0.0248

MarkerSeek score 72.40

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 46
pi: 0.0248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	CAAAAACAAAGCGGTCCCTC	55.0	50.0	58.1	57.9	1409-1481 bp	1.0	83.2
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.1	57.9	1412-1484 bp	1.0	83.0
trnT-ACC-psbD_p3	GGCGTAAGTCATCGGTTCAA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.3	57.9	1386-1458 bp	1.0	81.5
trnT-ACC-psbD_p4	TGGCCCTTTTAACTCAGTGG	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.7	57.9	1425-1497 bp	1.0	81.4
trnT-ACC-psbD_p5	GGCCCTTTTAACTCAGTGGT	CAAAAACAAAGCGGTCCCTC	50.0	50.0	57.7	57.9	1424-1496 bp	1.0	81.3