Hotspot

rpoB-trnC-UGC

LSC · 26044-27013 · 970 bp

Back to Rhammatophyllum

Pi 0.0304
MarkerSeek score 79.80
Primer available yes
Label rpoB-trnC-UGC

Feature Metadata

label_name
rpoB-trnC-UGC
feature_type
igs
region
LSC
length_bp
970
variable_sites
43
pi
0.0304
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-UGC_p1 GTTCCCTCTTTTCCATCCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 1058-1175 bp 1.0 82.3
rpoB-trnC-UGC_p2 GTTCCCTCTTTTCCATCCCC AAAAGGATTTGCAGTCCCCC 55.0 50.0 57.9 58.4 1040-1157 bp 1.0 80.2
rpoB-trnC-UGC_p3 GTTCCCTCTTTTCCATCCCC TCAAGTCTGTTGTTGAGGCG 55.0 50.0 57.9 58.4 1082-1199 bp 1.0 79.6
rpoB-trnC-UGC_p4 TGTTCCCTCTTTTCCATCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.1 58.1 1059-1176 bp 1.0 76.3
rpoB-trnC-UGC_p5 GTTCCCTCTTTTCCATCCCC AAGTCTGTTGTTGAGGCGG 55.0 52.6 57.9 58.0 1080-1197 bp 1.0 74.9