Hotspot

trnR-AGA-atpA

LSC · 9373-9680 · 308 bp

Back to Rhammatophyllum

Pi 0.0414

MarkerSeek score 75.10

Primer available yes

Label trnR-AGA-atpA

Feature Metadata

label_name: trnR-AGA-atpA
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 18
pi: 0.0414
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-AGA-atpA_p1	GCGAAAAGCGTCCATTGTC	ACATTAACCGCTGAAGCAGA	52.6	45.0	58.0	57.5	452-471 bp	1.0	75.3
trnR-AGA-atpA_p2	GCGAAAAGCGTCCATTGTC	AGACATTAACCGCTGAAGCA	52.6	45.0	58.0	57.5	454-473 bp	1.0	75.3
trnR-AGA-atpA_p3	GCGAAAAGCGTCCATTGTC	GACATTAACCGCTGAAGCAG	52.6	50.0	58.0	57.2	453-472 bp	1.0	72.6
trnR-AGA-atpA_p4	GCGAAAAGCGTCCATTGTC	TAACCGCTGAAGCAGAAAGC	52.6	50.0	58.0	58.8	448-467 bp	1.0	72.4
trnR-AGA-atpA_p5	GCGAAAAGCGTCCATTGTC	ACCAAGACATTAACCGCTGA	52.6	45.0	58.0	57.1	458-477 bp	1.0	72.3