Hotspot

psbT

LSC · 98951-99052 · 102 bp

Back to Reynoutria

Pi 0.0196

MarkerSeek score 63.80

Primer available yes

Label psbT

Feature Metadata

label_name: psbT
feature_type: gene
region: LSC
length_bp: 102
variable_sites: 2
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT_p1	CCCAATAAACAGGTATGGAAGC	GAGATCCGTTCGAGGAACAC	45.5	55.0	57.1	58.1	250 bp	1.0	56.6
psbT_p2	TCCCCAATAAACAGGTATGGA	GAGATCCGTTCGAGGAACAC	42.9	55.0	56.0	58.1	252 bp	1.0	56.0
psbT_p3	CCCAATAAACAGGTATGGAAGC	AGAGATCCGTTCGAGGAACA	45.5	50.0	57.1	58.2	251 bp	1.0	56.0
psbT_p4	TCCCCAATAAACAGGTATGGA	AGAGATCCGTTCGAGGAACA	42.9	50.0	56.0	58.2	253 bp	1.0	55.4
psbT_p5	CCCAATAAACAGGTATGGAAGC	ACACGGGGACTAGTTGAAGT	45.5	50.0	57.1	58.3	234 bp	1.0	55.0