Hotspot

ndhF

SSC · 139665-141908 · 2244 bp

Back to Reynoutria

Pi 0.00535

MarkerSeek score 45.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 12
pi: 0.00535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCGAACCTGCTCGTATACCT	AGCCGGTGAATCGAAAATCA	50.0	45.0	58.0	57.9	2992 bp	1.0	79.1
ndhF_p2	TCGAACCTGCTCGTATACCT	AAGAGCCGGTGAATCGAAAA	50.0	45.0	58.0	57.8	2995 bp	1.0	78.6
ndhF_p3	CGAACCTGCTCGTATACCTG	AGCCGGTGAATCGAAAATCA	55.0	45.0	57.9	57.9	2991 bp	1.0	78.5
ndhF_p4	CGAACCTGCTCGTATACCTG	AGAGGTAAGAGCCGGTGAAT	55.0	50.0	57.9	58.1	3000 bp	1.0	78.3
ndhF_p5	TCGAACCTGCTCGTATACCT	GAGGTAAGAGCCGGTGAATC	50.0	55.0	58.0	57.8	3000 bp	1.0	78.2