Hotspot

trnN-GUU-trnR-ACG

SSC · 159807-160412 · 606 bp

Back to Reynoutria

Pi 0.0066

MarkerSeek score 62.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 606
variable_sites: 4
pi: 0.0066
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	747 bp	1.0	78.7
trnN-GUU-trnR-ACG_p2	AAAATCCCCCGTTGTTCCTC	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	755 bp	1.0	78.1
trnN-GUU-trnR-ACG_p3	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	711 bp	1.0	77.5
trnN-GUU-trnR-ACG_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	698 bp	1.0	77.5
trnN-GUU-trnR-ACG_p5	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	699 bp	1.0	77.1