Hotspot

rpl32-trnL-UAG

SSC · 143145-143924 · 780 bp

Back to Reynoutria

Pi 0.0104

MarkerSeek score 61.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 780
variable_sites: 8
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCGAAATCTCTTTCTACCGG	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.8	57.9	867-877 bp	1.0	71.3
rpl32-trnL-UAG_p2	AGCGAAATCTCTTTCTACCGG	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.8	57.8	860-870 bp	1.0	70.9
rpl32-trnL-UAG_p3	AGCGAAATCTCTTTCTACCGG	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.8	57.8	866-876 bp	1.0	70.8
rpl32-trnL-UAG_p4	AGCGAAATCTCTTTCTACCGG	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.8	57.8	865-875 bp	1.0	70.8
rpl32-trnL-UAG_p5	AGAAAAGGATATTCGGCAGCA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	57.7	57.9	905-915 bp	1.0	70.3