Hotspot

ndhF-rpl32

SSC · 141909-142985 · 1077 bp

Back to Reynoutria

Pi 0.0112

MarkerSeek score 63.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1077
variable_sites: 12
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGAAGAAGTCCTGCTCCGAT	TGCTGCCGAATATCCTTTTCT	50.0	42.9	58.1	57.7	1243-1244 bp	1.0	69.5
ndhF-rpl32_p2	AGAAGAAGTCCTGCTCCGAT	AAAGCTTTTAATGCTGCCGA	50.0	40.0	58.1	56.6	1254-1255 bp	1.0	68.5
ndhF-rpl32_p3	AGAAGAAGTCCTGCTCCGAT	AAGCTTTTAATGCTGCCGAA	50.0	40.0	58.1	56.6	1253-1254 bp	1.0	68.5
ndhF-rpl32_p4	AGAAGAAGTCCTGCTCCGAT	GCAAAAGCTTTTAATGCTGCC	50.0	42.9	58.1	57.5	1257-1258 bp	1.0	68.0
ndhF-rpl32_p5	AAGAAGAAGTCCTGCTCCGA	TGCTGCCGAATATCCTTTTCT	50.0	42.9	58.4	57.7	1244-1245 bp	1.0	67.7