Hotspot

trnM-CAU-atpE

LSC · 76257-76523 · 267 bp

Back to Reynoutria

Pi 0.0187
MarkerSeek score 50.30
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
267
variable_sites
5
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGCAGGAGTCATTGGTTCAA AGAGGCTATCAATATGATTTCGT 50.0 34.8 58.1 55.4 327 bp 1.0 41.2
trnM-CAU-atpE_p2 TTGCTTTCATACGGCAGGAG AGAGGCTATCAATATGATTTCGT 50.0 34.8 58.3 55.4 339 bp 1.0 41.2
trnM-CAU-atpE_p3 CATACGGCAGGAGTCATTGG AGAGGCTATCAATATGATTTCGT 55.0 34.8 58.4 55.4 332 bp 1.0 41.2
trnM-CAU-atpE_p4 ATTGCTTTCATACGGCAGGA AGAGGCTATCAATATGATTTCGT 45.0 34.8 57.6 55.4 340 bp 1.0 41.2
trnM-CAU-atpE_p5 GGCAGGAGTCATTGGTTCAA GAGGCTATCAATATGATTTCGT 50.0 36.4 58.1 53.9 326 bp 1.0 41.2