Hotspot

rps4-trnT-UGU

LSC · 70067-70686 · 620 bp

Back to Reynoutria

Pi 0.00814

MarkerSeek score 64.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 5
pi: 0.00814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCCAGACGTCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	732-736 bp	1.0	79.3
rps4-trnT-UGU_p2	CCCCCAGACGTCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.9	58.2	701-705 bp	1.0	79.2
rps4-trnT-UGU_p3	CCCCCAGACGTCGTATTTTT	TTTATGTGAGCCCGCTTAGC	50.0	50.0	57.9	58.3	751-755 bp	1.0	77.6
rps4-trnT-UGU_p4	TAAAACCCCCAGACGTCGTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	737-741 bp	1.0	77.1
rps4-trnT-UGU_p5	TAAAACCCCCAGACGTCGTA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.4	58.2	706-710 bp	1.0	77.0