Hotspot

ycf3-trnS-GGA

LSC · 68112-69060 · 949 bp

Back to Reynoutria

Pi 0.00636

MarkerSeek score 63.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 6
pi: 0.00636
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCGCCTGAAGTTGTTGGAA	TGTTGGTGAGTTGGGTGAAG	45.0	50.0	58.0	57.9	1153-1155 bp	1.0	80.1
ycf3-trnS-GGA_p2	TTTCGCCTGAAGTTGTTGGA	TGTTGGTGAGTTGGGTGAAG	45.0	50.0	58.0	57.9	1154-1156 bp	1.0	80.1
ycf3-trnS-GGA_p3	TTCGCCTGAAGTTGTTGGAA	GGTCAACGATGTTGGTGAGT	45.0	50.0	58.0	58.1	1162-1164 bp	1.0	79.5
ycf3-trnS-GGA_p4	TTTCGCCTGAAGTTGTTGGA	GGTCAACGATGTTGGTGAGT	45.0	50.0	58.0	58.1	1163-1165 bp	1.0	79.5
ycf3-trnS-GGA_p5	TTGGCCACAACTGAAAAGGT	TGTTGGTGAGTTGGGTGAAG	45.0	50.0	58.1	57.9	1116-1118 bp	1.0	79.3