Hotspot

trnS-GCU-trnG-UCC

LSC · 30462-31528 · 1067 bp

Back to Reynoutria

Pi 0.0113

MarkerSeek score 66.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1067
variable_sites: 12
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	1189-1190 bp	1.0	51.8
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	1192-1193 bp	1.0	50.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	1191-1192 bp	1.0	50.8
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	1190-1191 bp	1.0	49.9
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	1175-1176 bp	1.0	48.1