Hotspot

rpl2-trnH-GUG

IRb · 20674-21055 · 382 bp

Back to Reynoutria

Pi 0.0123

MarkerSeek score 45.40

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRb
length_bp: 382
variable_sites: 1
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.21
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GGGCCCCAATTGGTAGAAAA	CGAAATAGGGGCGGATGTAG	50.0	55.0	58.1	57.9	585 bp	0.5	58.9
rpl2-trnH-GUG_p2	GGGCCCCAATTGGTAGAAAA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.1	58.1	574 bp	0.5	58.5
rpl2-trnH-GUG_p3	GGGCCCCAATTGGTAGAAAA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.1	57.9	560 bp	0.5	58.5
rpl2-trnH-GUG_p4	GGGCCCCAATTGGTAGAAAA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.1	57.9	559 bp	0.5	58.5
rpl2-trnH-GUG_p5	GGGCCCCAATTGGTAGAAAA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.1	57.9	561 bp	0.5	58.5