Hotspot

ycf1

SSC · 121754-126958 · 5205 bp

Back to Reseda

Pi 0.039
MarkerSeek score 61.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5205
variable_sites
203
pi
0.039
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TTTGCCCACGTAATCTGAGG GTCTTCAACCTTGGCACAGA 50.0 50.0 57.9 58.0 1183 bp 1.0 82.9
ycf1_p2 TTTGCCCACGTAATCTGAGG GATTGGAAACGCGAAATGCA 50.0 45.0 57.9 58.0 1301 bp 1.0 82.6
ycf1_p3 CGATTTCGACAGTTTTCGCC GTCTTCAACCTTGGCACAGA 50.0 50.0 58.1 58.0 1210 bp 1.0 82.6
ycf1_p4 CGATTTCGACAGTTTTCGCC GATTGGAAACGCGAAATGCA 50.0 45.0 58.1 58.0 1328 bp 1.0 82.4
ycf1_p5 TGCCCACGTAATCTGAGGTA GATTGGAAACGCGAAATGCA 50.0 45.0 58.1 58.0 1299 bp 1.0 82.3