Hotspot

rps15-ycf1

SSC · 121340-121753 · 414 bp

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Pi 0.0793
MarkerSeek score 77.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
414
variable_sites
31
pi
0.0793
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CGATTAGCCGAAGAACACCA CCTCAAAACGCCGTAGAGAA 50.0 50.0 58.0 57.9 1165 bp 0.5 59.4
rps15-ycf1_p2 ACGATTAGCCGAAGAACACC CCTCAAAACGCCGTAGAGAA 50.0 50.0 58.0 57.9 1166 bp 0.5 59.4
rps15-ycf1_p3 GGTGCTGAGGTCCCATATTG CCTCAAAACGCCGTAGAGAA 55.0 50.0 58.0 57.9 1135 bp 0.5 59.2
rps15-ycf1_p4 CGATTAGCCGAAGAACACCA AGTTCGTCTCGCTGGAAAAA 50.0 45.0 58.0 57.8 1383 bp 0.5 58.1
rps15-ycf1_p5 ACGATTAGCCGAAGAACACC AGTTCGTCTCGCTGGAAAAA 50.0 45.0 58.0 57.8 1384 bp 0.5 58.1