Hotspot

rpl32-trnL-UAG

SSC · 111499-112081 · 583 bp

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Pi 0.126
MarkerSeek score 81.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
583
variable_sites
72
pi
0.126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGGAACGGAAAGTGGAATG GAGCGCCATAGCCTAATACC 50.0 55.0 58.1 58.0 1843-1997 bp 1.0 84.4
rpl32-trnL-UAG_p2 TGGGAACGGAAAGTGGAATG AAAAGCCCCGAAGTAGCAAA 50.0 45.0 58.1 58.0 1748 bp 0.5 59.2
rpl32-trnL-UAG_p3 TGGGAACGGAAAGTGGAATG AAAGCCCCGAAGTAGCAAAA 50.0 45.0 58.1 58.0 1747 bp 0.5 59.2
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT AAAAGCCCCGAAGTAGCAAA 45.0 45.0 57.8 58.0 1218 bp 0.5 58.5
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT AAAGCCCCGAAGTAGCAAAA 45.0 45.0 57.8 58.0 1217 bp 0.5 58.5