Hotspot

petB-petD

LSC · 75304-75501 · 198 bp

Back to Reseda

Pi 0.0969
MarkerSeek score 75.80
Primer available yes
Label petB-petD

Feature Metadata

label_name
petB-petD
feature_type
igs
region
LSC
length_bp
198
variable_sites
19
pi
0.0969
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB-petD_p1 AGCAAGGTATTTCTGGTCCT TAGTTCAAGTCGCACACTCC 45.0 50.0 56.1 57.6 245-247 bp 1.0 68.3
petB-petD_p2 AGCAAGGTATTTCTGGTCCTT TAGTTCAAGTCGCACACTCC 42.9 50.0 56.9 57.6 245-247 bp 1.0 66.6
petB-petD_p3 AAGCAAGGTATTTCTGGTCCT TAGTTCAAGTCGCACACTCC 42.9 50.0 56.9 57.6 246-248 bp 1.0 66.5
petB-petD_p4 AGCAAGGTATTTCTGGTCCT CTTAGACGGTGGTTGGAACA 45.0 50.0 56.1 57.5 330-332 bp 1.0 65.7
petB-petD_p5 AGCAAGGTATTTCTGGTCCTT CTTAGACGGTGGTTGGAACA 42.9 50.0 56.9 57.5 330-332 bp 1.0 64.0