Hotspot

trnE-UUC-trnT-GGU

LSC · 30891-31272 · 382 bp

Back to Reseda

Pi 0.0707
MarkerSeek score 70.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
382
variable_sites
27
pi
0.0707
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TCCTGACCACTAGACGATGG GAACCGATGACTTACGCCTT 55.0 50.0 58.2 58.0 463-908 bp 1.0 81.3
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 486-931 bp 1.0 81.0
trnE-UUC-trnT-GGU_p3 TCCTGACCACTAGACGATGG CCATGGCGTTACTCTACCAC 55.0 55.0 58.2 58.1 442-887 bp 1.0 81.0
trnE-UUC-trnT-GGU_p4 TCCTGACCACTAGACGATGG TGGCGTTACTCTACCACTGA 55.0 50.0 58.2 58.1 439-884 bp 1.0 80.8
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 465-910 bp 1.0 80.6