Hotspot

trnC-GCA-petN

LSC · 27450-28200 · 751 bp

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Pi 0.0511
MarkerSeek score 81.60
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
751
variable_sites
37
pi
0.0511
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCGAGATTTACT 50.0 50.0 58.1 58.0 788-809 bp 1.0 85.5
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTT CAGCCCAAGCGAGATTTACT 50.0 50.0 58.4 58.0 806-827 bp 1.0 83.2
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 848-869 bp 1.0 82.5
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 866-887 bp 1.0 80.2
trnC-GCA-petN_p5 CCAGTTCAAATCCGGGTGTC CAGCCCAAGCGAGATTTACT 55.0 50.0 58.8 58.0 783-804 bp 1.0 79.5