Hotspot

rpoB-trnC-GCA

LSC · 26382-27377 · 996 bp

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Pi 0.0383
MarkerSeek score 66.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
996
variable_sites
38
pi
0.0383
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGAGCCCATATCCTTGCTTT CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1570-1588 bp 1.0 81.6
rpoB-trnC-GCA_p2 CGAGCCCATATCCTTGCTTT ATCAGCAAAGCAGAGTAGGC 50.0 50.0 58.0 58.0 1618 bp 0.5 59.5
rpoB-trnC-GCA_p3 CGAGCCCATATCCTTGCTTT AAGCAGAGTAGGCGATTTCG 50.0 50.0 58.0 58.1 1611 bp 0.5 59.2
rpoB-trnC-GCA_p4 CGAGCCCATATCCTTGCTTT GCAGAGTAGGCGATTTCGAA 50.0 50.0 58.0 58.1 1609 bp 0.5 59.2
rpoB-trnC-GCA_p5 CGAGCCCATATCCTTGCTTT ATGTCTGAGCACTCCGAAAC 50.0 50.0 58.0 57.9 1955 bp 0.5 59.1