Hotspot

atpF-atpH

LSC · 12680-13241 · 562 bp

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Pi 0.0486
MarkerSeek score 84.90
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
562
variable_sites
27
pi
0.0486
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CGGATGTCCAGTGATCCAAG AGGCAGAAGGAAAAATCCGA 55.0 45.0 58.1 57.1 727-749 bp 1.0 76.2
atpF-atpH_p2 TTAAATCCGAAACTCCCGGC AGGCAGAAGGAAAAATCCGA 50.0 45.0 58.3 57.1 746-768 bp 1.0 74.5
atpF-atpH_p3 GGATGTCCAGTGATCCAAGG AGGCAGAAGGAAAAATCCGA 55.0 45.0 57.7 57.1 726-748 bp 1.0 73.9
atpF-atpH_p4 CGGATGTCCAGTGATCCAAG GCAGAAGGAAAAATCCGAGG 55.0 50.0 58.1 56.8 725-747 bp 1.0 73.6
atpF-atpH_p5 CGGATGTCCAGTGATCCAAG GGCAGAAGGAAAAATCCGAG 55.0 50.0 58.1 56.8 726-748 bp 1.0 73.5