Hotspot

trnS-GCU-trnG-UCC

LSC · 8162-8647 · 486 bp

Back to Reseda

Pi 0.0557
MarkerSeek score 76.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
486
variable_sites
27
pi
0.0557
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGACCCCTTAACTATTCAGGG 50.0 52.4 58.0 57.1 587-604 bp 1.0 68.9
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA GGGACCCCTTAACTATTCAGG 50.0 52.4 58.0 57.1 588-605 bp 1.0 68.8
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC GGACCCCTTAACTATTCAGGG 50.0 52.4 58.0 57.1 586-603 bp 1.0 68.8
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGGACCCCTTAACTATTCAGG 50.0 52.4 58.0 57.1 587-604 bp 1.0 68.8
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC GGACCCCTTAACTATTCAGGG 50.0 52.4 58.4 57.1 589-606 bp 1.0 66.0