Hotspot

psbI-trnS-GCU

LSC · 8000-8071 · 72 bp

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Pi 0.0571
MarkerSeek score 76.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
72
variable_sites
4
pi
0.0571
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAAGA TGGACTAAAGCGTCGGATTG 50.0 50.0 57.1 58.0 169-170 bp 1.0 75.3
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAAGA GTGGACTAAAGCGTCGGATT 50.0 50.0 57.1 58.0 170-171 bp 1.0 75.3
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAAGA AGTTAGTCGTACCGAGGGTT 50.0 50.0 57.1 57.8 134-135 bp 1.0 74.4
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAAGA ATTGGGAGAGATGGCTGAGT 50.0 50.0 57.1 58.1 188-189 bp 1.0 74.2
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGA GAGTTAGTCGTACCGAGGGT 50.0 55.0 57.1 58.3 135-136 bp 1.0 74.0