Hotspot

rps16-trnQ-UUG

LSC · 6412-6849 · 438 bp

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Pi 0.0594
MarkerSeek score 73.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
438
variable_sites
26
pi
0.0594
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 511-512 bp 1.0 82.3
rps16-trnQ-UUG_p2 CCTTCAATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 521-522 bp 1.0 81.5
rps16-trnQ-UUG_p3 TCAATTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 518-519 bp 1.0 78.1
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 515-516 bp 1.0 77.6
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 503-504 bp 1.0 77.2