Hotspot

trnK-UUU-rps16

LSC · 4288-5234 · 947 bp

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Pi 0.0584
MarkerSeek score 77.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
947
variable_sites
54
pi
0.0584
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCCGGATCAGTCATGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1149-1170 bp 1.0 85.1
trnK-UUU-rps16_p2 CCTTTCCGGATCAGTCATGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1287-1308 bp 1.0 84.4
trnK-UUU-rps16_p3 CCTTTCCGGATCAGTCATGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 1288-1309 bp 1.0 84.4
trnK-UUU-rps16_p4 TCCGGATCAGTCATGGTCTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 1145-1166 bp 1.0 84.4
trnK-UUU-rps16_p5 CCTTTCCGGATCAGTCATGG AGTAAGGGCTTGTTGCTGTC 55.0 50.0 58.0 58.1 1800 bp 0.5 58.9