Hotspot

psaC-rps15

SSC · 113560-115409 · 1850 bp

Back to Renanthera

Pi 0.0319

MarkerSeek score 69.60

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 1850
variable_sites: 100
pi: 0.0319
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	GGACAAGCGGATTCACATCT	ATCACAAAGGGGTCTACGAA	50.0	45.0	58.0	56.2	1768-2190 bp	1.0	73.1
psaC-rps15_p2	GGACAAGCGGATTCACATCT	TCACAAAGGGGTCTACGAAT	50.0	45.0	58.0	56.2	1767-2189 bp	1.0	73.1
psaC-rps15_p3	GTCCATCGGTTTCCAGTCTC	ATCACAAAGGGGTCTACGAA	55.0	45.0	58.0	56.2	2229-2661 bp	1.0	72.9
psaC-rps15_p4	GTCCATCGGTTTCCAGTCTC	TCACAAAGGGGTCTACGAAT	55.0	45.0	58.0	56.2	2228-2660 bp	1.0	72.9
psaC-rps15_p5	TATACCAGAAAAGCCCGTGC	TCACAAAGGGGTCTACGAAT	50.0	45.0	58.0	56.2	1950-2372 bp	1.0	72.9