Hotspot

petD-rpoA

LSC · 78466-78637 · 172 bp

Back to Renanthera

Pi 0.0446

MarkerSeek score 74.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 14
pi: 0.0446
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GTACCGCAGTAGCTCTTTGG	TTGAGCACTGTACAGAAGCA	55.0	45.0	58.4	57.1	312-323 bp	1.0	73.4
petD-rpoA_p2	GGTACCGCAGTAGCTCTTTG	TTGAGCACTGTACAGAAGCA	55.0	45.0	58.4	57.1	313-324 bp	1.0	73.3
petD-rpoA_p3	CCGCAGTAGCTCTTTGGTTA	TTGAGCACTGTACAGAAGCA	50.0	45.0	57.6	57.1	309-320 bp	1.0	73.2
petD-rpoA_p4	TGGTTAGGGATTGGAGCAAC	TTGAGCACTGTACAGAAGCA	50.0	45.0	57.5	57.1	295-306 bp	1.0	72.4
petD-rpoA_p5	GTACCGCAGTAGCTCTTTGG	TGAGCACTGTACAGAAGCAT	55.0	45.0	58.4	56.9	311-322 bp	1.0	71.5