Hotspot

clpP-psbB

LSC · 72098-72793 · 696 bp

Back to Renanthera

Pi 0.0459

MarkerSeek score 80.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 56
pi: 0.0459
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	58.0	58.0	870-911 bp	1.0	89.0
clpP-psbB_p2	CGCACTATACGTCAACCCAA	AAGCAAGCGATTCCTGAGAG	50.0	50.0	58.0	58.0	1224-1265 bp	1.0	86.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1277-1318 bp	1.0	86.4
clpP-psbB_p4	CGCACTATACGTCAACCCAA	TAGACCCGCTAATATGCCCA	50.0	50.0	58.0	58.0	1414-1455 bp	1.0	85.8
clpP-psbB_p5	CGCACTATACGTCAACCCAA	TAGCTGCCAAGAAGCACAAA	50.0	45.0	58.0	58.0	1106-1118 bp	0.5	62.4