Hotspot

trnE-UUC-trnT-GGU

LSC · 32959-33840 · 882 bp

Back to Renanthera

Pi 0.0378
MarkerSeek score 79.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
882
variable_sites
49
pi
0.0378
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 888-986 bp 1.0 86.8
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 867-965 bp 1.0 86.4
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.8 58.1 864-962 bp 1.0 86.2
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 866-964 bp 1.0 86.0
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 885-983 bp 1.0 85.6