Hotspot

trnG-GCC-trnR-UCU

LSC · 10582-10721 · 140 bp

Back to Renanthera

Pi 0.0381

MarkerSeek score 70.40

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 8
pi: 0.0381
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	207-220 bp	1.0	70.4
trnG-GCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	210-223 bp	1.0	70.1
trnG-GCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	213-226 bp	1.0	69.6
trnG-GCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	216-229 bp	1.0	69.4
trnG-GCC-trnR-UCU_p5	CCAACGTCGACTATAACCCC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.8	56.9	228-241 bp	1.0	68.6