Hotspot

psbB-psbT

LSC · 73503-73700 · 198 bp

Back to Reichardia

Pi 0.00938

MarkerSeek score 47.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 4
pi: 0.00938
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGTGCTCGAACCTTGTTTAGA	CCCTAGAGTCGAGACTAAGAGG	47.6	54.5	58.2	58.3	358-369 bp	1.0	54.0
psbB-psbT_p2	GGTGCTCGAACCTTGTTTAG	CCCTAGAGTCGAGACTAAGAGG	50.0	54.5	56.7	58.3	358-369 bp	1.0	53.9
psbB-psbT_p3	GGTGCTCGAACCTTGTTTAGA	CCTAGAGTCGAGACTAAGAGGA	47.6	50.0	58.2	57.3	357-368 bp	1.0	50.7
psbB-psbT_p4	GGTGCTCGAACCTTGTTTAGA	TCCCTAGAGTCGAGACTAAGAG	47.6	50.0	58.2	57.3	359-370 bp	1.0	50.7
psbB-psbT_p5	GGTGCTCGAACCTTGTTTAG	CCTAGAGTCGAGACTAAGAGGA	50.0	50.0	56.7	57.3	357-368 bp	1.0	50.6