Hotspot

ndhC-trnV

LSC · 50210-51388 · 1179 bp

Back to Reichardia

Pi 0.00713

MarkerSeek score 52.40

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 20
pi: 0.00713
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TCCTTTGCTACTCGGAGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1321-1341 bp	1.0	80.3
ndhC-trnV_p2	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1312-1332 bp	1.0	79.0
ndhC-trnV_p3	TTCTCCGGTCCTTTGCTACT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	1329-1349 bp	1.0	78.5
ndhC-trnV_p4	TTTCTCCGGTCCTTTGCTAC	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.5	58.1	1330-1350 bp	1.0	77.7
ndhC-trnV_p5	CTCGGAGCTAAAAATCCCGA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1311-1331 bp	1.0	76.7