Hotspot

ycf3-N4L36_pgt015

LSC · 44017-44939 · 923 bp

Back to Reichardia

Pi 0.00449

MarkerSeek score 50.00

Primer available yes

Label ycf3-N4L36_pgt015

Feature Metadata

label_name: ycf3-N4L36_pgt015
feature_type: igs
region: LSC
length_bp: 923
variable_sites: 10
pi: 0.00449
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-N4L36_pgt015_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	1031-1033 bp	1.0	71.4
ycf3-N4L36_pgt015_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	981-983 bp	1.0	71.1
ycf3-N4L36_pgt015_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	1019-1021 bp	1.0	70.9
ycf3-N4L36_pgt015_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	983-985 bp	1.0	70.6
ycf3-N4L36_pgt015_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	1009-1011 bp	1.0	69.5