Hotspot

N4L36_pgt004-trnC

LSC · 8615-9349 · 735 bp

Back to Reichardia

Pi 0.00777

MarkerSeek score 47.90

Primer available yes

Label N4L36_pgt004-trnC

Feature Metadata

label_name: N4L36_pgt004-trnC
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 14
pi: 0.00777
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
N4L36_pgt004-trnC_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	884-887 bp	1.0	79.6
N4L36_pgt004-trnC_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	866-869 bp	1.0	77.4
N4L36_pgt004-trnC_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	820-823 bp	1.0	75.8
N4L36_pgt004-trnC_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	885-888 bp	1.0	75.4
N4L36_pgt004-trnC_p5	ACGGAAAGAGAGGGATTCGA	AAATCCCCTGTTGATGAGGC	50.0	50.0	58.2	57.9	911 bp	0.125	42.6