Hotspot

ndhA

SSC · 119815-121878 · 2064 bp

Back to Rehmannia

Pi 0.00582

MarkerSeek score 51.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2064
variable_sites: 23
pi: 0.00582
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAGACAAGCTGTGAAAATAGACT	GCCGGGTTTTATCAACTTGC	37.5	50.0	58.1	58.0	2168-2211 bp	1.0	49.5
ndhA_p2	TGAGACAAGCTGTGAAAATAGAC	GCCGGGTTTTATCAACTTGC	39.1	50.0	56.8	58.0	2168-2211 bp	1.0	49.2
ndhA_p3	TGAGACAAGCTGTGAAAATAGACT	CGCCGGGTTTTATCAACTTG	37.5	50.0	58.1	57.7	2169-2212 bp	1.0	47.6
ndhA_p4	TGAGACAAGCTGTGAAAATAGAC	CGCCGGGTTTTATCAACTTG	39.1	50.0	56.8	57.7	2169-2212 bp	1.0	47.2
ndhA_p5	TTTGAGACAAGCTGTGAAAA	GCCGGGTTTTATCAACTTGC	35.0	50.0	53.6	58.0	2170-2213 bp	1.0	47.2