Hotspot

rrn23-trnA(UGC)

IRa · 134108-134274 · 167 bp

Back to Rehmannia

Pi 0.018

MarkerSeek score 59.80

Primer available yes

Label rrn23-trnA(UGC)

Feature Metadata

label_name: rrn23-trnA(UGC)
feature_type: igs
region: IRa
length_bp: 167
variable_sites: 6
pi: 0.018
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-trnA(UGC)_p1	GTCTCTGGGTGCCTAGGTAT	GTTCGAGTCCGCTTATCTCC	55.0	55.0	57.9	57.9	224-240 bp	1.0	84.2
rrn23-trnA(UGC)_p2	TCGATTACTACGCCCTTCCT	GGTTCGAGTCCGCTTATCTC	50.0	55.0	57.9	57.9	246-262 bp	1.0	83.9
rrn23-trnA(UGC)_p3	TCGATTACTACGCCCTTCCT	GTTCGAGTCCGCTTATCTCC	50.0	55.0	57.9	57.9	245-261 bp	1.0	83.9
rrn23-trnA(UGC)_p4	ATTCGGGAATCTCCGGATCT	GGTTCGAGTCCGCTTATCTC	50.0	55.0	58.0	57.9	297-313 bp	1.0	83.4
rrn23-trnA(UGC)_p5	ATTCGGGAATCTCCGGATCT	GTTCGAGTCCGCTTATCTCC	50.0	55.0	58.0	57.9	296-312 bp	1.0	83.4