Hotspot

ndhG-ndhI

SSC · 118873-119233 · 361 bp

Back to Rehmannia

Pi 0.0327

MarkerSeek score 76.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 26
pi: 0.0327
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	CGGTTACCGGTGTCAATAATTG	50.0	45.5	58.0	57.7	476-496 bp	1.0	69.8
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	CGGTTACCGGTGTCAATAAT	50.0	45.0	58.0	55.6	476-496 bp	1.0	68.4
ndhG-ndhI_p3	TCCCAATGAAAAGGCCGAAT	CGGTTACCGGTGTCAATAATTG	45.0	45.5	57.8	57.7	539-559 bp	1.0	67.6
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	CGGTTACCGGTGTCAATAATT	50.0	42.9	58.0	56.3	476-496 bp	1.0	66.6
ndhG-ndhI_p5	TCCCAATGAAAAGGCCGAAT	CGGTTACCGGTGTCAATAAT	45.0	45.0	57.8	55.6	539-559 bp	1.0	66.2