Hotspot

rps12-trnV(GAC)

IRb · 99086-100700 · 1615 bp

Back to Rehmannia

Pi 0.0111
MarkerSeek score 56.50
Primer available yes
Label rps12-trnV(GAC)

Feature Metadata

label_name
rps12-trnV(GAC)
feature_type
igs
region
IRb
length_bp
1615
variable_sites
38
pi
0.0111
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV(GAC)_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1711-1757 bp 1.0 78.9
rps12-trnV(GAC)_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1715-1761 bp 1.0 78.8
rps12-trnV(GAC)_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1710-1756 bp 1.0 78.8
rps12-trnV(GAC)_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1713-1759 bp 1.0 77.9
rps12-trnV(GAC)_p5 TGGCAACTTTACGTAAGGCA GTCAAGGTGACACTCTACCG 45.0 55.0 57.7 57.7 1696-1742 bp 1.0 77.4