Hotspot

psbE-petL

LSC · 66231-66940 · 710 bp

Back to Rehmannia

Pi 0.031

MarkerSeek score 77.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 48
pi: 0.031
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.9	55.9	855-1073 bp	1.0	69.9
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.8	55.9	778-996 bp	1.0	69.7
psbE-petL_p3	ACTGACGAATAACCAACCCG	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.9	55.9	868-1086 bp	1.0	69.5
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGCCACTAGTAGAAAACCGA	45.0	45.0	58.2	55.9	864-1082 bp	1.0	68.8
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.8	55.9	863-1081 bp	1.0	68.7