Hotspot

cemA-petA

LSC · 63186-63426 · 241 bp

Back to Rehmannia

Pi 0.0277

MarkerSeek score 60.40

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 14
pi: 0.0277
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CGTGTATCTCCGTCACTTGT	GCACTTGAAATGGATGTCCG	50.0	50.0	57.4	57.5	380-404 bp	1.0	76.7
cemA-petA_p2	TCGTGTATCTCCGTCACTTG	GCACTTGAAATGGATGTCCG	50.0	50.0	57.1	57.5	381-405 bp	1.0	74.4
cemA-petA_p3	ATCGTGTATCTCCGTCACTTG	GCACTTGAAATGGATGTCCG	47.6	50.0	57.6	57.5	382-406 bp	1.0	70.4
cemA-petA_p4	CGTGTATCTCCGTCACTTGT	ATCAGGAGCGATACGGAAAT	50.0	45.0	57.4	56.5	347-371 bp	1.0	69.7
cemA-petA_p5	CGTGTATCTCCGTCACTTGT	CGCAAAAATGGGATATGCACT	50.0	42.9	57.4	57.6	396-420 bp	1.0	69.3