Hotspot

trnT(UGU)-trnL(UAA)

LSC · 47410-47990 · 581 bp

Back to Rehmannia

Pi 0.01

MarkerSeek score 66.20

Primer available yes

Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name: trnT(UGU)-trnL(UAA)
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 15
pi: 0.01
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(UGU)-trnL(UAA)_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	625-824 bp	1.0	82.7
trnT(UGU)-trnL(UAA)_p2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	676-875 bp	1.0	81.8
trnT(UGU)-trnL(UAA)_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	657-856 bp	1.0	81.7
trnT(UGU)-trnL(UAA)_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	619-818 bp	1.0	81.4
trnT(UGU)-trnL(UAA)_p5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	657-856 bp	1.0	80.5