Hotspot

psbZ-trnG(GCC)

LSC · 36526-36816 · 291 bp

Back to Rehmannia

Pi 0.0349

MarkerSeek score 57.30

Primer available yes

Label psbZ-trnG(GCC)

Feature Metadata

label_name: psbZ-trnG(GCC)
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 23
pi: 0.0349
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG(GCC)_p1	ACCCGTCGTATTTGCTTCTC	AATCCTGTCTCCGCAACATC	50.0	50.0	58.0	58.0	634-704 bp	1.0	87.0
psbZ-trnG(GCC)_p2	ACCCGTCGTATTTGCTTCTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	688-758 bp	1.0	86.8
psbZ-trnG(GCC)_p3	ACCCGTCGTATTTGCTTCTC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	961-1031 bp	1.0	86.2
psbZ-trnG(GCC)_p4	TCCATTTCCCCTGACTCCAT	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1200-1270 bp	1.0	85.0
psbZ-trnG(GCC)_p5	CAACCACTCAGCCATCTCTC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.0	58.0	1384-1454 bp	1.0	84.4