Hotspot

trnS(GCU)-trnG(UCC)

LSC · 8565-9311 · 747 bp

Back to Rehmannia

Pi 0.00656

MarkerSeek score 52.30

Primer available yes

Label trnS(GCU)-trnG(UCC)

Feature Metadata

label_name: trnS(GCU)-trnG(UCC)
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 12
pi: 0.00656
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnG(UCC)_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	793-850 bp	1.0	56.6
trnS(GCU)-trnG(UCC)_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	792-849 bp	1.0	56.5
trnS(GCU)-trnG(UCC)_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	796-853 bp	1.0	55.5
trnS(GCU)-trnG(UCC)_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	795-852 bp	1.0	55.5
trnS(GCU)-trnG(UCC)_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	794-851 bp	1.0	55.5