Hotspot

ycf1

SSC · 68021-73525 · 5505 bp

Back to Rehderodendron

Pi 0.00182

MarkerSeek score 53.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5505
variable_sites: 23
pi: 0.00182
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTGGTTTTGTGCATTTGATCT	ACCGTGGTTTATACCTAGCA	34.8	45.0	57.5	56.0	189-195 bp	1.0	41.7
ycf1_p2	TCTTGGTTTTGTGCATTTGATCT	AACCGTGGTTTATACCTAGCA	34.8	42.9	57.5	56.7	190-196 bp	1.0	41.7
ycf1_p3	TCTTGGTTTTGTGCATTTGATCT	ACCGTGGTTTATACCTAGCAA	34.8	42.9	57.5	56.7	189-195 bp	1.0	41.7
ycf1_p4	TCTTGGTTTTGTGCATTTGA	ACCGTGGTTTATACCTAGCA	35.0	45.0	54.2	56.0	189-195 bp	1.0	41.7
ycf1_p5	TCTTGGTTTTGTGCATTTGATC	ACCGTGGTTTATACCTAGCA	36.4	45.0	56.1	56.0	189-195 bp	1.0	41.7