Hotspot

petD

LSC · 21229-22444 · 1216 bp

Back to Rehderodendron

Pi 0.00148

MarkerSeek score 54.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1216
variable_sites: 4
pi: 0.00148
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATATTTCCCTTCAACCCCGC	GGGAGAATTCACTTTGAAGCG	50.0	47.6	57.9	57.5	1369-1415 bp	1.0	67.9
petD_p2	CCCTTCAACCCCGCAATATT	GGGAGAATTCACTTTGAAGCG	50.0	47.6	58.2	57.5	1363-1409 bp	1.0	66.6
petD_p3	ATATTTCCCTTCAACCCCGC	ACTTTGAAGCGACTATTCCCT	50.0	42.9	57.9	57.0	1359-1405 bp	1.0	63.8
petD_p4	ATATTTCCCTTCAACCCCGC	CACTTTGAAGCGACTATTCCC	50.0	47.6	57.9	57.0	1360-1406 bp	1.0	63.5
petD_p5	ATATTTCCCTTCAACCCCGC	GGAGAATTCACTTTGAAGCGAC	50.0	45.5	57.9	57.9	1368-1414 bp	1.0	63.1