Hotspot

trnT-GGU-psbD

LSC · 130388-131805 · 1418 bp

Back to Rehderodendron

Pi 0.00183

MarkerSeek score 43.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1418
variable_sites: 6
pi: 0.00183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1568-1634 bp	1.0	77.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1571-1637 bp	1.0	77.6
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1567-1633 bp	1.0	76.6
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	AGAGCAATAAACCCGACCAG	55.0	50.0	58.1	57.6	1591-1657 bp	1.0	76.6
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1570-1636 bp	1.0	76.5