Hotspot

rbcL-accD

LSC · 1429-2073 · 645 bp

Back to Rehderodendron

Pi 0.00372

MarkerSeek score 46.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 6
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CAACATCGAATTGAACCGCC	55.0	50.0	58.0	58.1	816-854 bp	1.0	79.4
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	CAACATCGAATTGAACCGCC	50.0	50.0	57.9	58.1	786-824 bp	1.0	78.9
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	AACATCGAATTGAACCGCCT	55.0	45.0	58.0	58.2	815-853 bp	1.0	78.8
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCGCCTT	55.0	45.0	58.0	58.2	814-852 bp	1.0	78.8
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	ACATCGAATTGAACCGCCTT	50.0	45.0	57.9	58.2	784-822 bp	1.0	78.3